| ORIGINAL ARTICLE |
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| Year : 2023 | Volume
: 14
| Issue : 1 | Page : 49 |
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Protective effect of vitamin D supplementation on some hemogram derived inflammatory indices in normal and high-fat diet fed male wistar rats
Fahimeh Agh1, Seyed H Mousavi2, Naheed Aryaeian1, Fatemehsadat Amiri1, Mohammad R Jalilvand3, Leila Janani4, Motahareh Hasani5, Fatemeh Sepahvand6, Fahimeh Zamani-Garmsiri7
1 Department of Nutritions, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
2 Department of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
3 Natural products and medicinal plants research center, North Khorasan University of Medical Sciences, Bojnurd, Iran
4 Department of Biostatsitics, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
5 Department of Nutrition, School of Health, Golestan University of Medical Sciences, Golestan, Iran
6 Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
7 Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Correspondence Address:
Naheed Aryaeian
Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran
Iran
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/ijpvm.ijpvm_505_20
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Background: Hematological inflammatory indices are currently suggested to assess systemic inflammation. This study aims to investigate a vitamin D supplementation effect on hematological indices of inflammation in rats. Method: Forty-eight middle-aged male rats were allocated into a normal diet (ND) group (10% fat) and a high-fat diet (HFD) group (60% fat). The animals were fed for 26 weeks. After this period, each group was randomly divided into three subgroups, each of 8 rats: Group (1): animals were fed the ND and HFD containing 1 IU/g vitamin D for 4 months, group (2): animals were fed the ND and HFD containing 6 IU/g vitamin D for 4 months and group (3): animals were euthanized to evaluate the HFD effect. Serum 25-hydroxyvitamin D level, white blood cell count (WBCs), platelet count, platelet crit (PCT), mean platelet volume (MPV), platelet distribution width (PDW), platelet-to-lymphocyte ratio (PLR), neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR) were measured. Results: The HFD, significantly increased body weight, PCT, PDW, PLR, NLR, and MLR and significantly reduced serum vitamin D levels compared to the ND (P < 0.05). There was a significant decrease in food intake, MPV, PDW, and NLR after vitamin D supplementation in the ND-fed group (P < 0.05). A significant reduction in platelet count, PCT, and MLR was observed after vitamin D supplementation in HFD-fed rats (P < 0.05). Conclusions: In our study, some hemogram-derived inflammatory indices were higher in the HFD-fed group, and vitamin D supplementation lowering effects on some hematological indices were seen in both ND and HFD groups.
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