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Year : 2015  |  Volume : 6  |  Issue : 1  |  Page : 67

Competitive protein-binding assay-based enzyme-immunoassay method, compared to high-pressure liquid chromatography, has a very lower diagnostic value to detect vitamin D deficiency in 9-12 years children

Department of Laboratory of Nutrition Research, National Research Institute, Faculty of Nutritional Science and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Correspondence Address:
Tirang Reza Neyestani
No. 7, Hafezi St., Farahzadi Blvd., Qods Town, Tehran
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Source of Support: National Research Institute, Faculty of Nutritional Science and Food Technology, Conflict of Interest: None

DOI: 10.4103/2008-7802.161590

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Background: The most reliable indicator of Vitamin D status is circulating concentration of 25-hydroxycalciferol (25(OH) D) routinely determined by enzyme-immunoassays (EIA) methods. This study was performed to compare commonly used competitive protein-binding assays (CPBA)-based EIA with the gold standard, high-pressure liquid chromatography (HPLC). Methods: Concentrations of 25(OH) D in sera from 257 randomly selected school children aged 9-11 years were determined by two methods of CPBA and HPLC. Results: Mean 25(OH) D concentration was 22 ± 18.8 and 21.9 ± 15.6 nmol/L by CPBA and HPLC, respectively. However, mean 25(OH) D concentrations of the two methods became different after excluding undetectable samples (25.1 ± 18.9 vs. 29 ± 14.5 nmol/L, respectively; P = 0.04). Based on predefined Vitamin D deficiency as 25(OH) D < 12.5 nmol/L, CPBA sensitivity and specificity were 44.2% and 60.6%, respectively, compared to HPLC. In receiver operating characteristic curve analysis, the best cut-offs for CPBA was 5.8 nmol/L, which gave 82% sensitivity, but specificity was 17%. Conclusions: Though CPBA may be used as a screening tool, more reliable methods are needed for diagnostic purposes.

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